Guiseley Association Football Club is a professional football club based in Guiseley, West Yorkshire, England. They are currently members of the National League, the fifth tier of English football, and play at Nethermoor Park.

The club was established in 1909 and initially played in the Wharfedale League. They were league champions in 1912–13, and after World War I the club transferred to the Leeds League. In 1924 they moved leagues again, this time joining the West Riding County Amateur League. They went on to win three consecutive league titles between 1932–33 and 1934–35, before finishing as runners-up in 1937–38 and then winning the league again in 1938–39.